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Image Search Results
Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology
Article Title: Changes in intestinal homeostasis and immunity in a cigarette smoke- and LPS-induced murine model for COPD: the lung-gut axis
doi: 10.1152/ajplung.00486.2021
Figure Lengend Snippet: Inflammatory markers in serum. Mice were exposed to air or cigarette smoke for 72 days, except on days 42 , 52 , and 62 . On these days mice were treated with saline or lipopolysaccharide (LPS) via intratracheal instillation. Serum was collected and the concentration of inflammatory mediators was determined. C-reactive protein (CRP; A ), keratinocyte-derived chemokine (KC; B ), and VEGF-A ( C ) levels were detected by using an ELISA and Milliplex Luminex assay kit, respectively. Values are expressed as means ± SE. * P < 0.05; cigarette smoke (CS) group compared with air group. Correlations between KC levels in bronchoalveolar lavage (BAL) fluid and KC levels in serum ( D ) were analyzed by using Spearman correlation. Air + vehicle: n = 12 animals; air + LPS: n = 13 animals; CS + vehicle: n = 12 animals; CS + LPS: n = 13 animals.
Article Snippet: C-reactive protein (CRP) levels in the BAL fluid and serum were measured by ELISA (
Techniques: Saline, Concentration Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay, Luminex
Journal: Physiological Reports
Article Title: CFTR dysfunction increases endoglin and TGF‐ β signaling in airway epithelia
doi: 10.14814/phy2.13977
Figure Lengend Snippet: Increased endoglin in CF bronchoalveolar lavage fluid ( BAL ) fluid. Quantitative analysis by human CD 105 ELISA kit (Cat.# EK 0644, Boster Immuno‐Leader Biotechnology) indicates a fivefold increase in endoglin in severe CF ( n = 14) compared to non‐ CF samples (* P < 0.05, n = 5; A ). Immunohistochemistry on paraffin‐embedded lung sections shows increased ENG (brown staining) in CF lungs with severe disease as compared to non‐ CF (100x; primary Ab: Rabbit polyclonal anti‐human ENG , 1:50 dilution, Santa Cruz, Calibration bar = 250 μ m) (B).
Article Snippet: Quantitative analysis of ENG levels was measured in
Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining